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产品英文名称: WNT-5a, Human
产品中文名称:人重组WNT-5a蛋白
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WNT-5a, Human产品介绍:
Wnt信号转导通路因其启动蛋白为Wnt蛋白而得名,在胚胎发育、细胞增殖及分化等方面发挥重要 作用,其异常活化与人类多种肿瘤的发生密切相关。研究显示,Wnt信号至少存在经典和非经典两条不同的信号通路,而Wnt-5a属于非经典Wnt信号通 路,既往研究Ror2具有Wnt受体结构域样结构。有研究认为Wnt-5a经由Ror2介导的 信号通路发挥作用。
Human WNT-5a蛋白含有357个氨基酸残基。
WNT-5a, Human 产品特性:
» 活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定,纯度高,无动物成分污染
» WNT-5a, Human活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定
» WNT-5a, Human产品经过SDS-PAGE 和HPLC双层鉴定纯度大于85%
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WNT-5a, Human产品英文简述:
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical" pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.
? Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.
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StemRD MGro-500 MSC medium,chemically-defined,间充质干细胞培养基 500mL/瓶
LONZA 04-418Q X-VIVO 15 无血清培养基 1L/瓶
Life Technology 087-0112DK AIM-V? CTS? 无血清细胞培养基(细胞治疗级) 1000mL/瓶
Pall 15950-017 Ultroser Serum Substitute 血清替代品 20mL/瓶
LONZA 12-725F UltraCULTURE Serum-free Medium无血清培养基 500mL/瓶
BD 762165 PAXgene Blood RNA Tube RNA样本收集管 100管/盒
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LONZA CC-3162 EGM-2 BulletKit( CC-3156 & CC-4176 )套装 Kit/套
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Lonza 50-650U Kinetic-QCL 192 Test Kit内毒素检测试剂盒 192T/盒
Corning 431080 175cm⒉细胞培养瓶,Angled颈,透气盖 50个/箱
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BD Falcon 352070 50mL锥形离心管,高透明度聚丙烯,袋装 500支/箱
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BD BioCoat 354234 Matrigel Basement Membrane Matrix(标准型) 10mL/瓶
Cygnus F550 CHO HCP ELISA Kit,3G宿主蛋白检测试剂盒 96T/盒
Cygnus F410 E.coli HCP ELISA Kit 大肠杆菌 96T/盒
Nalgene 5100-0001 Cryo 1℃ Freezing Container梯度降温冻存盒 1个/箱
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Hyclone SH30084.03 FBS 优等澳洲胎牛血清 500mL/瓶
PeproTech 100-18B-500 Recombinant Human FGF-basic (154 a.a.)因子 500ug/支
Parafilm PM-996 Parafilm? M Laboratory Film 封口膜 12卷/箱